The aim of speed training is to condition the athlete to move at high velocity, employing maximal power when needed. In order to do this, the neuromuscular system must be conditioned to very fast movements and training need to be very specific, with a very high anaerobic component (Martin & Coe, 1991). Sprint training is an effective training method designed to elicit enhancements in motor fitness and physiological parameters. It has vastly different training effects depending upon the intensity and duration of the work and rest period. More research is required concerning the variation in different methods of sprint training and their effect. For the purpose of this study, fifteen untrained male students of Annamalai University in the age group of eighteen to twenty years were recruited, with their consent. The selected subjects were healthy and normal, and they were physically fit enough to undergo the sprint training programme. The selected dependent variables namely Total Cholesterol, High Density Lipoprotein Cholesterol (HDL-C), Low Density Lipoprotein Cholesterol (LDL-C) and Triglycerides were assessed using standard tests and procedures, prior to and immediately after the training protocol. Estimation of lipoprotein variables was done with the help of a Biochemist and laboratory technicians. The speed training programme conducted for a period of eight weeks in the study indicated a positive change in total cholesterol and low-density lipoprotein cholesterol level in case of male students.