The present of this study was to compare of depression among the male and female athletes of Mumbai University Mumbai and Gondwana University Gadchiroli, Maharashtra state. A group of total 120 players (30 male athletes and 30 female athletes of both universities) were randomly selected from Krida Mahotsav competition during the year 2012-13 to 2014-15. Prof. L. N. Dubey’s mental depression test was used to collect the data. Data were analyzed by independent ‘t’ test using by Statistics Open for All (SOFA). By analyzing the proposed hypothesis at the 0.01 demonstrated no significant difference between male and female athletes of Mumbai University Mumbai and Gondwana University Gadchiroli in depression.